Fundamentals of Recombinant Protein Production, Purification and Characterization

1. Introduction to recombinant protein expression and purification
2. Recombinant protein expression in Escherichia coli
3. Recombinant protein expression in Pichia pastoris
4. Mammalian cells for recombinant protein production via transient gene transfer
5. Expression of recombinant proteins in Chlamydomonas reinhardtii
6. Extraction and concentration of proteins (Laboratory scale)
7. Chromatographic and non-chromatographic techniques for purifying recombinant proteins
8. Analysis for purity of recombinant proteins
9. Characterization of recombinant proteins

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• Biotecnologia
• Biologia molecular

protein purification; protein expression; gene transfer; recombinant proteins; Protein corona; Surface modification; metal ion affinity; Functionalized polymers; Carbon nanomaterials; antibodies